Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS HTR1E β-Arrestin Cell Line, 2 vials. (93-1030C3)
The PathHunter HTR1E cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR1E GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1E and EA-tagged β-Arrestin-2. Activation of HTR1E stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS mINSRa Functional Assay, 2 vials. (93-1015C3)
The PathHunter U2OS mINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Lonza Walkersville Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKit
Stem cells possess the unique ability to differentiate into multiple lineages of cells including osteoblasts. Lonza’s hMSC Osteogenic Differentiation BulletKit Medium is a serum containing medium designed to induce osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hMSC) into mature, functionally active osteoblasts in as few as 14 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s OsteoImage Bone Mineralization Reagent which provides a quantitative value related to the amount of osteogenic differentiation.Content:1 x hMSC Osteogenic Differentiation Basal Medium (PT-3924), 170 mL1 x hMSC Osteogenic Differentiation SingleQuots Supplements Kit (PT-4120)
Lonza Walkersville HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM-2
Lonza's Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XL guaranteed through 5 PD).All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.Benefits:Guaranteed through 15 population doublings Test negative for mycoplasma, bacteria, yeast, and fungiHIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lotsA Certificate of Analysis is provided for each cell lot purchased Applications:Wound healingAngiogenesisTissue engineeringInflammationOncologyPharmacologyVascular modelingTransfection Content:1 x Cryopreserved ampule of HUVEC containing ≥ 500,000 cells
Genscript Corporation CHO-K1/NPS1b/G15 Stable Cell
Recombinant CHO-K1 cells stably overexpress human neuropeptide S receptor 1 (NPSR1) isoform B on the surface and contain high levels of G protein G s and G q to couple with the receptor in downstream signaling pathways
Eurofins Discoverx PathHunter U2OS GHR-JAK1 Functional Assay, 2 vials. (93-0756C3)
The PathHunter GHR-JAK1 Functional assay cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting CTKs. This cell line enables assessment of GHR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter BDKRB2 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0725C3)
The PathHunter BDKRB2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of BDKRB2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged BDKRB2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting BDKRB2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS HTR1B β-Arrestin Cell Line, 2 vials. (93-0697C3)
The PathHunter HTR1B cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1B and EA-tagged β-Arrestin-2. Activation of HTR1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS OPRM1 β-Arrestin-1 Cell Line, 2 vials. (93-0878C3)
The PathHunter OPRM1 (ARR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. met 5-enkephalin) based activation of OPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRM1 and EA-tagged β-Arrestin-1. Activation of OPRM1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
iXCells Biotechnologies Peripheral Blood Mononuclear Cells (PBMCs), 100M
Peripheral Blood Mononuclear Cells (PBMCs) is any blood cell with a single nucleus. PBMCs consist of lymphocytes (T, B and NK cells), monocytes and dendritic cells. iXCells Biotechnologies offers PBMCs from the human peripheral blood of individual healthy donors. These PBMCs are purified from peripheral blood using ficoll gradient centrifugation.
iXCells Biotechnologies Peripheral Blood Mononuclear Cells (PBMCs), 50M
Peripheral Blood Mononuclear Cells (PBMCs) is any blood cell with a single nucleus. PBMCs consist of lymphocytes (T, B and NK cells), monocytes and dendritic cells. iXCells Biotechnologies offers PBMCs from the human peripheral blood of individual healthy donors. These PBMCs are purified from peripheral blood using ficoll gradient centrifugation.